When employing non‐scorching‐commence Taq polymerase, it's best to transfer the reactions immediately from ice to the PCR block pre‐heated to 50°C, without the need of permitting them to take a seat at home temperature. Especially, we identified the SARS‐CoV‐2 N1 primer set was very delicate to area temperature incubation, whilst https://www.pinterest.com/pin/993958580252567868/